Life and Environmental Sciences
Reverse transcriptase (RT) PCR
PHR2: FAD binding domain of DNA photolyase family protein; utilizes blue light to repair DNA function
The PHR2 (photolyase repair) gene functions in the repair of DNA in Tetrahymena thermophila. It was hypothesized that when Tetrahymena cultures are exposed to UV light, increased expression of PHR2 would occur in response to the damage incurred. UV light has been known to cause the formation of pyrimidine dimers and other disruptions in DNA. Through two rounds of experimentation, the acute impact of UV light exposure was monitored. The cultures underwent RNA extraction, reverse transcription, and semi-quantitative PCR analysis. We predicted that UV exposure would result in the increased expression of PHR2.
Moloney, Molly; Gustavsen, Tanner; and Otto-Hitt, Stefanie, "UV Light Exposure and Tetrahymena thermophilia: Expression of the PHR2 Gene" (2018). Life and Environmental Sciences Course-based Research Projects. 16.