Publication Date

Summer 2018

Document Type

Paper

Department

Life and Environmental Sciences

First Instructor

Stefanie Otto-Hitt

Experiment Type

Reverse transcriptase (RT) PCR

Feature

TTHERM 00571860

Gene

ATP1: ATP synthase F1, alpha subunit family protein

Abstract

In this study, we investigated the effect of temperature on expression of the ATP1 gene in the organism Tetrahymena thermophila. We hypothesized that expression of the ATP1 gene would increase as we increased temperature. ATP1 is a highly conserved gene in Tetrahymena that codes for the alpha subunit of ATP Synthase, an enzyme that generates the energy molecule ATP. We decided to use heat treatment based on studies performed on ATP1 in Alaskan Cod showing increased expression in response to increased temperatures (Michael et al., 2015). We used reverse transcription and semi-quantitative PCR to analyze expression of ATP1 in our heat-treated Tetrahymena cultures. We predicted that ATP1 expression would be up-regulated as temperature increased.

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