Date of Award

Spring 1983

Document Type



Life & Environmental Sciences

First Advisor

James Manion

Second Advisor

Guido Bugni

Third Advisor

Joseph Ward


The formation of a partial blastema in adult mice was achieved through treatment of the amputation surface of toes with saturated solution of sodium chloride and a one mM equimolar solution of db-cAMP and theophylline. This study represents an improvement on the study first successful in stimulating partial blastema formation in mice (Neufeld, 1980) . The innovation employed in this study involved the use of db-cAMP and theophylline as an inhibitor of epidermal cell migration (epidermal migration onto the amputation plane inhibits regeneration). The advantages of chemical inhibition over surgical removal of epidermal tissue is discussed.