Date of Award

Spring 1983

Document Type

Thesis

Department

Life & Environmental Sciences

First Advisor

John Christenson

Second Advisor

James Manion

Third Advisor

Rev. Jeremiah Sullivan

Abstract

Methods of preparing chromosomes from human lymphocytes for viewing with the scanning electron microscope (SEM) were investigated. Several fixatives were used. Chromosomes were isolated on nuclear pore filters, critical point dried, and coated with gold for SEM. Chromosome spreads were not very clear and chromosome overlap prevented establishment of clean, complete karyotypes.

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