Date of Award
Life & Environmental Sciences
The methods of isolating human and rat large granular lymphocytes were reviewed, and revised to accommodate horse lymphocytes. Purified populations of large granular lymphocytes were then stimulated with Interleukin-2 and Concanavalin-A, to observe effects on proliferation and cytotoxic activity. The results of this study suggest that large granular lymphocytes can be purified from the peripheral blood of horses, using a percoll density gradient. Also, Interleukin-2 is shown to have an effect on proliferation of enriched populations of large granular lymphocytes, especially when added in adjunct to Concanavalin-A.
MacNeill, Susan, "Isolation Of Horse Large Granular Lymphocytes By Density Centrifugation Techniques, And Responsiveness To Human Interleukin-2" (1985). Life and Environmental Sciences Undergraduate Theses. 393.