Date of Award

Spring 1986

Document Type


First Advisor

James Manion

Second Advisor

Arthur Westwell

Third Advisor

Joseph Vantura


Pertussigen is a toxin responsible for the ADP-ribosylation of the Adenylate cyclase system inhibitory component. It has been verified through polyacrylamide gel electrophoresis that the ADP-ribosylation occurs only if the reaction mixture is supplemented with ATP, NAD and DTT. There is also a linear relationship between time and the amount of protein which is ADP ribosylated. Pertussigen can also ADP-ribosylize Bovine Serum Albumin (BSA) which offers researchers an inexpensive way to study the action of pertussigen.