Date of Award

Spring 1986

Document Type



Life & Environmental Sciences

First Advisor

James Manion

Second Advisor

Jean Smith

Third Advisor

Ruth Carrington


After the isolation and fractionation of histones Hl and Hl" from lamb thymus and bovine liver, these chromosomal proteins were purified for further research and experimentation as to how this family of histones functions in chromatin. The relationship of the Hl" subtype of histone Hl with DNA lengths was studied using a preparation of mouse liver nuclei. The Hl" protein was found to be associated with longer lengths of DNA between 146 and 200 nucleotide base pairs.