Date of Award
Life & Environmental Sciences
Rev. Eugene Peoples
Chinese hamster ovary (CHO) cells were exposed to 60-Hz electric fields at 18 V/m and 5 V/m, and the cloning efficiencies compared. Statistical studies failed to show a significant dose response for the field values tested. However, with regard to the 5 V/m field, a trend of 85% viability compared to sham-exposed cells was discovered. In general, cloning efficiencies for sham-exposed controls were low. Various techniques were employed to increase the efficiencies of the controls. Suggestions for improving controls and the overall procedure are discussed.
Popp, Julie, "The Study Of The Effects Of 60-HZ Electric Fields On The Cloning Efficiencies Of Chinese Hamster Ovary Cells" (1987). Life and Environmental Sciences Undergraduate Theses. 353.