Date of Award

Spring 1988

Document Type

Thesis

Department

Life & Environmental Sciences

First Advisor

John Christenson

Second Advisor

Jean Smith

Third Advisor

Ruth Carrington

Abstract

Monoclonal antibodies specific for epitopes on the perstussigen molecule, s-1, were developed by the Fetuin-ELISA test, radioimmunoassays and Western Blott(27). Hybridoma fluids already established for specificity on s-2, s-4 and the undigested molecule were exposed to pertussigen to detect the neutralization of agglutination to Chinese Hamster Ovary (CHO) cells. The results showed that no appreciable neutralization occurred. Many more monoclonals need to be developed and tested to find the critical epitope or epitopes which cause agglutination to CHO cells. This research may provide a clue to the specific antigenic site which causes toxicity attributed to whooping cough in humans.

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