Date of Award

Spring 1989

Document Type

Thesis

Department

Life & Environmental Sciences

Abstract

As a first step leading toward construction of a mouse-human chimeric antibody against Group B streptococcus, attempts were made to clone the heavy variable region gene from a murine hybridoma. The hybridoma was known to produce an anti-group B streptococcus monoclonal antibody. A conventional phagevector cloning approach was utilized as were the polymerase chain reaction and DNA size differentiation by electrophoresis. Attempts were unsuccessful, but a hybridoma gene library was created.

Share

COinS