Date of Award
Life & Environmental Sciences
Rev. Jeremiah Lowney
Rev. Joseph Harrington
A clone of Dictyostelium discoideum strain AX-3 was cultured up to its amoeboid stage. The glycoproteins in the plasma membrane of strain AX-3 were then isolated and purified. The proteins were fractionated by SDS polyacrylamide gel electrophoresis and then transferred to a nitrocellulose sheet by western blotting. The resulting nitrocellulose blot was used to detect glycoproteins with biotinylated concanavalin A and avidin-alkaline phosphatase. The results suggest there are mannose containing glycoproteins in the plasma membrane of strain AX-3.
Wood, Chris, "The Detection Of Glycoproteins In The Plasma Membrane Of Dictyostelium discoideum" (1992). Life and Environmental Sciences Undergraduate Theses. 282.