Date of Award

Spring 1992

Document Type



Life & Environmental Sciences

First Advisor

John Christenson

Second Advisor

Rev. Jeremiah Lowney

Third Advisor

Rev. Joseph Harrington


A clone of Dictyostelium discoideum strain AX-3 was cultured up to its amoeboid stage. The glycoproteins in the plasma membrane of strain AX-3 were then isolated and purified. The proteins were fractionated by SDS polyacrylamide gel electrophoresis and then transferred to a nitrocellulose sheet by western blotting. The resulting nitrocellulose blot was used to detect glycoproteins with biotinylated concanavalin A and avidin-alkaline phosphatase. The results suggest there are mannose containing glycoproteins in the plasma membrane of strain AX-3.

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