Date of Award
Life & Environmental Sciences
Gadolinium, a Calcium-sensing Receptor (CaR) agonist, was applied in different concentrations to rat Superior Cervical Ganglia (SCG) to discover the dissociation constant and the maximum amount of CaR-mediated current that could be blocked by this agonist. It was hypothesized that these concentrations would be similar to those of previously determined concentrations for other neural tissues, suggesting a similar CaR complex shared by the tissues. The SCG were dissected from male rats, cultured, and neural activity was recorded through a modified patch-clamping technique. It was shown that gadolinium’s dissociation constant was in the hundreds of nanomolars (nM), and that the agonist could block all CaR-mediated current. Previous research has shown gadolinium blocking other important calcium channels, however, and ultimately a method of blocking only the current generated by the CaR is desired to gain control of excess neurological activity as seen in stroke, cerebral ischemia, and seizures.
Brennan, Maire, "Effects of Gadolinium on Calcium-Sensing Receptor (CaR)-mediated Calcium Current in Rat Superior Cervical Ganglia" (2003). Life and Environmental Sciences Undergraduate Theses. 237.