Date of Award

Spring 2004

Document Type



Life & Environmental Sciences

First Advisor

Kyle Strode

Second Advisor

Ron Wilde

Third Advisor

Debra Bernardi


Isocratic elution of aromatic amino acids was achieved using a butyl-imidazolium based stationary phase and UV detection. For this study butyl-imidazolium bromide was bonded to a silica substrate and packed into an HPLC column. This study focused on the effects of ionic strength and organic modifier on retention. Retention of aromatic dipeptides was also explored to gain further insight into the retention mechanisms exhibited by this new stationary phase. The stationary phase was found to exhibit both reverse-phase and ion-exchange retention mechanisms.