Topoisomerase II in the Inactive X Chromosome as Determined by Immunofluorescence

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Authors
Lehmann, Ronald
Advisor
John Addis
Marilyn Schendel
Philip Wittman
Editor
Date of Issue
1990-04-01
Subject Keywords
Publisher
Citation
Series/Report No.
item.page.identifier
Title
Topoisomerase II in the Inactive X Chromosome as Determined by Immunofluorescence
Other Titles
Type
thesis
Description
Abstract
Topoisomerases are enzymes that convert DNA from one topological state to another. A particular type of topoisomerase, type II, appears also to be a member of the chromosome scaffold, bound to the base of radial DNA loops. Using immunofluorescence coupled with DNase digestions, we determined an association between the topoisomerase II and the sex chromatin body. Based upon this association, we postulate that topoisomerase II may be involved in the compartmentalization of the sex chromatin body. Since no topoisomerase II has been detected in the G-] phase of the cell cycle, topoisomerase II must act with other, yet to be determined proteins in order to maintain the compartmentalization of the sex chromatin body throughout the cell cycle.
Sponsors
Degree Awarded
Bachelor's
Semester
Spring
Department
Life & Environmental Sciences