Construction of a chimeric antibody against group B streptococcus: Cloning the variable region of the mouse heavy chain gene

carrollscholars.legacy.contextkey12273634
carrollscholars.legacy.itemurlhttps://scholars.carroll.edu/lifesci_theses/321
carrollscholars.object.degreeBachelor's
carrollscholars.object.departmentLife & Environmental Sciences
carrollscholars.object.disciplinesImmunology and Infectious Disease; Immunology of Infectious Disease
carrollscholars.object.seasonSpring
dc.contributor.authorJohnson, Mike
dc.date.accessioned2020-04-30T10:02:05Z
dc.date.available2020-04-30T10:02:05Z
dc.date.embargo12/31/1899 0:00
dc.date.issued1989-04-01
dc.description.abstractAs a first step leading toward construction of a mouse-human chimeric antibody against Group B streptococcus, attempts were made to clone the heavy variable region gene from a murine hybridoma. The hybridoma was known to produce an anti-group B streptococcus monoclonal antibody. A conventional phagevector cloning approach was utilized as were the polymerase chain reaction and DNA size differentiation by electrophoresis. Attempts were unsuccessful, but a hybridoma gene library was created.
dc.identifier.urihttps://scholars.carroll.edu/handle/20.500.12647/3063
dc.titleConstruction of a chimeric antibody against group B streptococcus: Cloning the variable region of the mouse heavy chain gene
dc.typethesis
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