Construction of a chimeric antibody against group B streptococcus: Cloning the variable region of the mouse heavy chain gene
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Authors
Johnson, Mike
Date of Issue
1989-04-01
Type
thesis
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Abstract
As a first step leading toward construction of a mouse-human chimeric antibody against Group B streptococcus, attempts were made to clone the heavy variable region gene from a murine hybridoma. The hybridoma was known to produce an anti-group B streptococcus monoclonal antibody. A conventional phagevector cloning approach was utilized as were the polymerase chain reaction and DNA size differentiation by electrophoresis. Attempts were unsuccessful, but a hybridoma gene library was created.