Exploring the Role of Dync1li1 in the Trafficking of GLUA2 Containing AMPA Receptors
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Authors
Esposito, Leah
Date of Issue
2017-05-13
Type
thesis
Language
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Abstract
AMPA receptors are glutamate receptors that are found in the post-synaptic region of a neuron. They are one of the main receptors that are responsible for excitatory signaling in the central nervous system. The trafficking of these receptors is an area of knowledge that is not well developed, but is essential to a complete understanding of these receptor proteins. My research was focused on exploring the role that Dync1li1 has in the trafficking of GluA2-containing AMPARs. In order to investigate this role, the gene encoding Dync1li1 was amplified via PCR, cloned into a mammalian expression vector and transfected into HEK293 cells and NT2 neurons. Live labeling studies and Co-immunoprecipitation experiments were performed to determine association with GluA2 and surface expression. Immunostaining experiments were performed to view Dync1li1 localization. Although the quantifiable results were statistically insignificant, further experimentation is needed. In conclusion, the role that Dync1li1 plays in the trafficking of GluA2-containing AMPARs remains unclear.