Neutralization Of Agglutination On Chinese Hamster Ovary Cells By Pertussigen

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Authors
Vogelman, Lee
Advisor
John Christenson
Jean Smith
Ruth Carrington
Editor
Date of Issue
1988-04-01
Subject Keywords
Publisher
Citation
Series/Report No.
item.page.identifier
Title
Neutralization Of Agglutination On Chinese Hamster Ovary Cells By Pertussigen
Other Titles
Type
thesis
Description
Abstract
Monoclonal antibodies specific for epitopes on the perstussigen molecule, s-1, were developed by the Fetuin-ELISA test, radioimmunoassays and Western Blott(27). Hybridoma fluids already established for specificity on s-2, s-4 and the undigested molecule were exposed to pertussigen to detect the neutralization of agglutination to Chinese Hamster Ovary (CHO) cells. The results showed that no appreciable neutralization occurred. Many more monoclonals need to be developed and tested to find the critical epitope or epitopes which cause agglutination to CHO cells. This research may provide a clue to the specific antigenic site which causes toxicity attributed to whooping cough in humans.
Sponsors
Degree Awarded
Bachelor's
Semester
Spring
Department
Life & Environmental Sciences