Injury History and its Effect on the Metabolic Profile of Human Platelet- Poor Plasma (PPP)

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Authors
Crichton, Juliette
Kastner, Kaden
Kuga, Kylie
Advisor
Hahn, Alyssa
Editor
Date of Issue
2024
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Title
Injury History and its Effect on the Metabolic Profile of Human Platelet- Poor Plasma (PPP)
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Description
Abstract
In this advanced research experiment, metabolites in Platelet Poor Plasma (PPP) were extracted and analyzed between two cohorts; patients with previous injury and patients without previous injury. This experiment is aimed to aid healthcare professionals in facilitating better patient care and elevate the current understanding of metabolites within the field of orthopedics. PPP is a readily available byproduct found in humans, making it an advantageous medium to explore as it relates to healing and increased treatment effectiveness. It was hypothesized that differences in metabolic profile would exist between those cohorts based on the body's previous need to respond to trauma or lack thereof, especially in those metabolites necessary for regeneration. Metabolite extraction was performed in labs and then sent to Montana State University for Liquid chromatography mass spectroscopy. The resulting identified metabolites were then uploaded into Metaboanalyst where univariate and multivariate analysis were performed to determine if there were statistically significant differences between cohorts. These analyses were then run through pathway enrichment to identify the specific metabolites and metabolic pathways important in PPP.
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Biology