Isolation and Preliminary Characterization of Mitochondrial DNA From the Thatching Ant, Formica obscuripes

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Authors
Dover, Deborah
Advisor
John Addis
Marilyn Schendel
Art Westwell
Editor
Date of Issue
1995-04-01
Subject Keywords
thatching ant, Formica obscuripes
Publisher
Citation
Series/Report No.
item.page.identifier
Title
Isolation and Preliminary Characterization of Mitochondrial DNA From the Thatching Ant, Formica obscuripes
Other Titles
Type
thesis
Description
Abstract
A simple and relatively quick procedure was developed for the extraction of mitochondrial DNA from the thatching ant Formica obscuripes . DNA obtained using this procedure was analyzed both after digestion with restriction enzymes (Eco R1 and Bam H1) and without digestion by electrophoresis on agarose gels. Although the DNA isolated appeared to be larger than the usual upper size limit (approximately 20 kb) for mitochondrial DNA, the undigested and digested molecules behaved as would be expected of a circular molecule with one restriction enzyme recognition site (i.e., the undigested form migrated further into the gel than the digested form).
Sponsors
Degree Awarded
Bachelor's
Semester
Spring
Department
Life & Environmental Sciences