Linear Cell Mass Increase during the Cell Cycle of Escherichia coli

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Authors
Winslow, Patricia
Advisor
Rev. Joseph Harrington
John Christenson
Robert Swartout
Editor
Date of Issue
1986-04-01
Subject Keywords
Publisher
Citation
Series/Report No.
item.page.identifier
Title
Linear Cell Mass Increase during the Cell Cycle of Escherichia coli
Other Titles
Type
thesis
Description
Abstract
Linear increase of cell mass of undivided cells was determined during the division cycle of Escherichia coli B/rA. Synchronous growth was obtained by selection of cells from exponentially growing populations after velocity sedimentation in sucrose density gradients. Cell mass was calculated as a product of mean cell volume and buoyant density that were measured at intervals during synchronous growth. A liner increase in cell mass was observed in two different growth media which had doubling times of 40.0 and 30.1 minutes. The results support the theory of constant linear increase of cell mass throughout the division cycle.
Sponsors
Degree Awarded
Bachelor's
Semester
Spring
Department
Life & Environmental Sciences