ADP-ribosylation of BSA by Pertussigen Follows the same Mechanism as ADP-ribosylation of Transducine
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Authors
Cabrera, John
Date of Issue
1986-04-01
Type
thesis
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Abstract
Pertussigen is a toxin responsible for the ADP-ribosylation of the Adenylate cyclase system inhibitory component. It has been verified through polyacrylamide gel electrophoresis that the ADP-ribosylation occurs only if the reaction mixture is supplemented with ATP, NAD and DTT. There is also a linear relationship between time and the amount of protein which is ADP ribosylated. Pertussigen can also ADP-ribosylize Bovine Serum Albumin (BSA) which offers researchers an inexpensive way to study the action of pertussigen.