Global metabolic profiling of human joint fluid following a second ACL tear: A case report

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Authors
Starke, Jenna
Advisor
Alyssa Hahn
Brandon Sheafor
Kelly Parsley
Editor
Date of Issue
2019-01-01
Subject Keywords
ACL tear, metabolic profiling
Publisher
Citation
Series/Report No.
item.page.identifier
Title
Global metabolic profiling of human joint fluid following a second ACL tear: A case report
Other Titles
Type
thesis
Description
Abstract
Objectives: The objective of this study was to investigate the acute metabolic response to injury using global, or untargeted, metabolic profiling. The purpose was to better understand the acute response to injury following a second traumatic joint injury. Methods: This study is a case report of a patient who had sustained two separate ACL tears in the same knee years apart. We extracted metabolites from healthy synovial fluid and human joint fluid following the patient’s second ACL tear and analyzed the metabolite extracts using liquid chromatography-mass spectrometry (LC-MS)-based global metabolic profiling. Results: We identified 1241 unique metabolite features in injured SF compared to healthy SF (Student’s t-test: FDR-adjusted p-value
Sponsors
Degree Awarded
Bachelor's
Semester
Winter
Department
Health Sciences