In Situ Hybridization Of Complementary Deoxyribonucleic Acid Probes To The 1448-1455 Region Of Escherichia coli 16s Ribosomal Subunits

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Authors
Lubbers, Angela
Advisor
John Christenson
John Addis
Art Westwell
Editor
Date of Issue
1988-04-01
Subject Keywords
Publisher
Citation
Series/Report No.
item.page.identifier
Title
In Situ Hybridization Of Complementary Deoxyribonucleic Acid Probes To The 1448-1455 Region Of Escherichia coli 16s Ribosomal Subunits
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Type
thesis
Description
Abstract
30S subunits were isolated from Escherichia coli ribosomes and purified 16S RNA was subsequently extracted. An eight-base DNA oligomer [d(CCCGAAGG)] was hybridized in situ to the 1448-1455 region of the 16S rRNA. The cDNA-rRNA hybrid was digested with the RNA specific enzyme RNase H. The clipped RNA fragments were then separated by polyacrylamide gel electrophoresis. The desired RNA band was visualized by UV shadowing, eluted from the gel and sequenced to verify the specificity of the probe binding. The results suggest that the cDNA and rRNA were binding, but proof that it was specific to the 1448-1455 region is still lacking.
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Degree Awarded
Bachelor's
Semester
Spring
Department
Life & Environmental Sciences