AAV Mediated Alpha CaM-Kinase II Suppression and Up Regulation: Behavioral Studies and Western Blot Analysis

Loading...
Thumbnail Image
Authors
Robinson, Deven
Advisor
Jacqueline Brehe
John Addis
Jeffrey Morris
Editor
Date of Issue
2006-04-01
Subject Keywords
Enzymes , Memory disorders -- Animal models , Protein kinases , Proteins , Western immunoblotting
Publisher
Citation
Series/Report No.
item.page.identifier
Title
AAV Mediated Alpha CaM-Kinase II Suppression and Up Regulation: Behavioral Studies and Western Blot Analysis
Other Titles
Type
thesis
Description
Abstract
The goal of the present study was to both inhibit the a-CaM-Kinase II (cc-CaM-K II) enzyme, and to over-express it in neurons in order to observe if the enzyme has a role to play in memory as measured by behavioral and learning tests. An adeno-associated virus (AAV) containing either sense or anti-sense RNA to the a-CaM-K II protein was designed for the over-expression or suppression of the enzyme, respectively. Morriswater and open field mazes were used to test for the effects of the differences in protein concentration on memory and behavior. An increase in protein in the rats receiving sense RNA, as apparent in Western Blots, correlated with significantly greater time in the target quadrant when compared to the controls, as expected if a-CaM-K II protein was involved in the establishment of memory. Concentrations of a-CaM-K II protein in the hippocampi were determined 29 days after introduction of the virus using Western Blots. The concentrations of a-CaM-K II protein in the rats receiving sense RNA ( n = 6 ) were statistically larger (p
Sponsors
Degree Awarded
Bachelor's
Semester
Spring
Department
Life & Environmental Sciences