Separation of Underivatized Amino Acids and Dipeptides Using 1- Butylimidazolium Based Stationary Phase and UV Detection

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Authors
Blackburn, Amanda
Advisor
Kyle Strode
Ron Wilde
Debra Bernardi
Editor
Date of Issue
2004-04-01
Subject Keywords
Publisher
Citation
Series/Report No.
item.page.identifier
Title
Separation of Underivatized Amino Acids and Dipeptides Using 1- Butylimidazolium Based Stationary Phase and UV Detection
Other Titles
Type
thesis
Description
Abstract
Isocratic elution of aromatic amino acids was achieved using a butyl-imidazolium based stationary phase and UV detection. For this study butyl-imidazolium bromide was bonded to a silica substrate and packed into an HPLC column. This study focused on the effects of ionic strength and organic modifier on retention. Retention of aromatic dipeptides was also explored to gain further insight into the retention mechanisms exhibited by this new stationary phase. The stationary phase was found to exhibit both reverse-phase and ion-exchange retention mechanisms.
Sponsors
Degree Awarded
Bachelor's
Semester
Spring
Department
Life & Environmental Sciences