A Study Of Radiation-Induced Heritable Damage (Small-Colony-Formation) In Chinese Hamster Cells

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Authors
Tacke, Sam
Advisor
James Manion
John Christenson
Lindsay Hess
Editor
Date of Issue
1978-04-01
Subject Keywords
Publisher
Citation
Series/Report No.
item.page.identifier
Title
A Study Of Radiation-Induced Heritable Damage (Small-Colony-Formation) In Chinese Hamster Cells
Other Titles
Type
thesis
Description
Abstract
Seventeen small-colony-forming sublines of V79-AL885D were isolated and their radiosensitivities assayed. It was found that most sublines were more sensitive to UV and X-ray irradiation and more resistant to near-UV irradiation than the parental line. No correlation was found between ouabain resistance and small-colony-formation. An attempt was made to adapt the tetrazolium salt assay for respiration deficiency in yeasts to mammalian cell systems. Although color developed over most colonies, the ability of the test to reflect metabolic rates in V79 Chinese hamster cells was not conclusively demonstrated.
Sponsors
Degree Awarded
Bachelor's
Semester
Spring
Department
Life & Environmental Sciences