Preparation Of Chronic Lymphocytic Leukemic Chromosomes For Viewing With The Scanning Electron Microscope
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Authors
Tangen, Lorraine
Date of Issue
1983-04-01
Type
thesis
Language
Subject Keywords
Other Titles
Abstract
Methods of preparing chromosomes from human lymphocytes for viewing with the scanning electron microscope (SEM) were investigated. Several fixatives were used. Chromosomes were isolated on nuclear pore filters, critical point dried, and coated with gold for SEM. Chromosome spreads were not very clear and chromosome overlap prevented establishment of clean, complete karyotypes.