Preparation Of Chronic Lymphocytic Leukemic Chromosomes For Viewing With The Scanning Electron Microscope

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Authors
Tangen, Lorraine
Advisor
John Christenson
James Manion
Rev. Jeremiah Sullivan
Editor
Date of Issue
1983-04-01
Subject Keywords
Publisher
Citation
Series/Report No.
item.page.identifier
Title
Preparation Of Chronic Lymphocytic Leukemic Chromosomes For Viewing With The Scanning Electron Microscope
Other Titles
Type
thesis
Description
Abstract
Methods of preparing chromosomes from human lymphocytes for viewing with the scanning electron microscope (SEM) were investigated. Several fixatives were used. Chromosomes were isolated on nuclear pore filters, critical point dried, and coated with gold for SEM. Chromosome spreads were not very clear and chromosome overlap prevented establishment of clean, complete karyotypes.
Sponsors
Degree Awarded
Bachelor's
Semester
Spring
Department
Life & Environmental Sciences