Isolation and Analysis of the Novel Mycobacteriophage, CCP1
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Authors
McNamee, Kevin
Advisor
Editor
Date of Issue
2015-04-01
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Citation
Series/Report No.
item.page.identifier
Title
Isolation and Analysis of the Novel Mycobacteriophage, CCP1
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Type
thesis
Description
Abstract
Bacteriophages are the largest remaining body of undescribed biodiversity on the planet. The present study attempted to isolate and analyze the structure and genetics of a novel mycobacteriophage extracted from a soil sample. It was hypothesized that undescribed phages exist in the study area. A phage, named CCP1, was isolated from soil obtained in Helena, Montana, U.S.A. via serial plating of phage material taken from plaques formed in Mycobacterium smegmatis. Genetic and morphological analyses by restriction enzyme fragment length polymorphism (RFLP) analysis and transmission electron microscopy (TEM) respectively, suggest that CCP1 is a novel mycobacteriophage.
Sponsors
Degree Awarded
Bachelor's
Semester
Spring
Department
Life & Environmental Sciences