Methotrexate As A Teratogenic Substance On The Central Nervous System Of CD-1 Laboratory Mice

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Mitchell, Bradley

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1985-04-01

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This study evaluated the teratogenic potential of mathorexate on the central nervous system of CD-I mice at low concentrations during early gestation. Pregnant mice were given C.i mg <2.5 mg/kg) of methotrexate by intramuscular injections on day 5 of gestation while pregnant control mice received an injection of distilled water. Fetuses were excised by cesarian sectioning on days 7 through 20 of gestation and sectioned for histological examination. Other mice from each group were allowed to go to term and the offspring were examined externally at 7 and 14 days of age. An increased mortality rate, a decrease in fetal size, and slight internal hydrocephaly were ail observed in some of the mice which were prenatally exposed to the methotrexate.

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