Variation In Mitochondrial DNA As A Means Of Analyzing Invertebrate Populations
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Authors
Bugni, James
Advisor
John Addis
Marilyn Schendel
Fr. William Greytak
Marilyn Schendel
Fr. William Greytak
Editor
Date of Issue
1993-04-01
Subject Keywords
Publisher
Citation
Series/Report No.
item.page.identifier
Title
Variation In Mitochondrial DNA As A Means Of Analyzing Invertebrate Populations
Other Titles
Type
thesis
Description
Abstract
Because of the similar properties of mitochondrial DNA and plasmid DNA, methods for the isolation of plasmid DNA can be adapted for the isolation of mitochondrial DNA. In the present study, a method for mitochondrial DNA isolation and analysis was used in an attempt to gain information about variability in a local population of snails (Helisoma sp.). DNA obtained from the foot of the snail was cleaved with the restriction endonuclease EcoR1, electrophoresed on agarose gels and stained. Although problems with the method prevented the principal goal of the project from being reached, a method was developed which should permit future analyses of genetic variability.
Sponsors
Degree Awarded
Bachelor's
Semester
Spring
Department
Life & Environmental Sciences