Classification And Initial Characterization Of Symbiotic Chlorella Sp. Isolated From The Freshwater Sponge, Ephydatia Muelleri
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Authors
Erickson, Michael
Advisor
John Addis
Jacqueline Brehe
Rev. Jeremiah Lowney
Jacqueline Brehe
Rev. Jeremiah Lowney
Editor
Date of Issue
1994-04-01
Subject Keywords
freshwater sponge, Ephydatia muelleri
Publisher
Citation
Series/Report No.
item.page.identifier
Title
Classification And Initial Characterization Of Symbiotic Chlorella Sp. Isolated From The Freshwater Sponge, Ephydatia Muelleri
Other Titles
Type
thesis
Description
Abstract
A population of archeocytes was isolated from the freshwater sponge, Ephydatia muelleri, using a Ficoll gradient. From these archeocytes an endozoic Chlorella sp. was removed and cultured. The cultured Chlorella sp. were then used to investigate the uptake of symbiotes by E. muelleri. The cultured algae were added to germinated gemmules. The outgrowth from the gemmules was then examined for algae uptake by the sponge cells using brightfield and phase contrast photomicroscopy. The results were inconclusive because algal symbiotes inhabited sponge cells of both the experimental group (the sponges exposed to the algae culture) and the control group (the sponges not exposed to algae culture).
Sponsors
Degree Awarded
Bachelor's
Semester
Spring
Department
Life & Environmental Sciences