The Study Of The Effects Of 60-HZ Electric Fields On The Cloning Efficiencies Of Chinese Hamster Ovary Cells

Abstract

Chinese hamster ovary (CHO) cells were exposed to 60-Hz electric fields at 18 V/m and 5 V/m, and the cloning efficiencies compared. Statistical studies failed to show a significant dose response for the field values tested. However, with regard to the 5 V/m field, a trend of 85% viability compared to sham-exposed cells was discovered. In general, cloning efficiencies for sham-exposed controls were low. Various techniques were employed to increase the efficiencies of the controls. Suggestions for improving controls and the overall procedure are discussed.