UV Light Exposure and Tetrahymena thermophilia: Expression of the PHR2 Gene

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Authors
Moloney, Molly
Gustavsen, Tanner
Otto-Hitt, Stefanie
Advisor
Stefanie Otto-Hitt
Editor
Date of Issue
2018-07-01
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Citation
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Title
UV Light Exposure and Tetrahymena thermophilia: Expression of the PHR2 Gene
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paper
Description
Abstract
The PHR2 (photolyase repair) gene functions in the repair of DNA in Tetrahymena thermophila. It was hypothesized that when Tetrahymena cultures are exposed to UV light, increased expression of PHR2 would occur in response to the damage incurred. UV light has been known to cause the formation of pyrimidine dimers and other disruptions in DNA. Through two rounds of experimentation, the acute impact of UV light exposure was monitored. The cultures underwent RNA extraction, reverse transcription, and semi-quantitative PCR analysis. We predicted that UV exposure would result in the increased expression of PHR2.
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Semester
Summer
Department
Life and Environmental Sciences