Prion infection of the nasal cavity: site for agent shedding?

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Authors
Moreaux, Brian
Advisor
Editor
Date of Issue
2006-04-01
Subject Keywords
prion disease, shedding, prion shedding
Publisher
Citation
Series/Report No.
item.page.identifier
Title
Prion infection of the nasal cavity: site for agent shedding?
Other Titles
Type
thesis
Description
Abstract
The mechanism of prion shedding in prion disease is currently unknown. The purpose of this study was to determine if the nasal cavity is a site of prion infection. Hamsters were intracerebrally inoculated with the hyper strain of transmissible mink encephalopathy agent. Once clinically ill, the hamsters were euthanized and their skulls were removed and examined for prion infection using immunohistochemical and dual immunofluorescence staining techniques. In infected hamsters, the infectious agent (PrP50) was shown to be present in the olfactory receptor neurons of the olfactory sensory epithelium (OSE) as well as in the nasal-associated lymphoid tissue and the vomeronasal receptor neurons in the vomeronasal sensory epithelium (VSE) of the vomeronasal organ. PrPSc infection was specific to sensory and not to non-sensory epithelium in both the OSE and the VSE.
Sponsors
Degree Awarded
Bachelor's
Semester
Spring
Department
Life & Environmental Sciences