Discrimination Between Two Types Of Freshwater Sponges Using Allozyme Electrophoresis

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Authors
Clark, Tricia
Advisor
John Addis
Guido Bugni
Joan Stottlemyer
Editor
Date of Issue
1998-04-01
Subject Keywords
freshwater sponge, species, allozyme electrophoresis
Publisher
Citation
Series/Report No.
item.page.identifier
Title
Discrimination Between Two Types Of Freshwater Sponges Using Allozyme Electrophoresis
Other Titles
Type
thesis
Description
Abstract
Allozyme electrophoresis was used to discriminate between two types of freshwater sponges that previously could not be distinguished using morphological criteria. One of these types was a well-characterized species, Ephydatia muelleri, whereas the other could not be identified because of an absence of gemmules and gemmoscleres, structures on which classification of freshwater sponges largely depends. Homogenates prepared from five individuals of each sponge type were electrophoresed in cellulose acetate gels. The gels were then stained to localize bands of enzyme activity. Analyzing the stained gels revealed migration rates of hexokinase and sorbitol dehydrogenase that differed between the two sponge types. The absence of any electrophoretic bands common to the two sponge types argues that the sponges are different species.
Sponsors
Degree Awarded
Bachelor's
Semester
Spring
Department
Life & Environmental Sciences