A Simple Staining Procedure for Quantifying Cell Adhesion in a Microtiter System

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Authors
Schmechel, Stephen
Advisor
John Addis
John Christenson
John Semmens
Editor
Date of Issue
1990-04-01
Subject Keywords
Publisher
Citation
Series/Report No.
item.page.identifier
Title
A Simple Staining Procedure for Quantifying Cell Adhesion in a Microtiter System
Other Titles
Type
thesis
Description
Abstract
The present study was designed to find a simple and effective staining technique to replace the tritiatedthymidine method in cell adhesion assays in 96-well microtiter plates. This method, a variation of previously used staining methods (Cardarelli and Pierschbacher, 1986; Barer, Lyon, and Draser, 1986), produced higher non-cellspecific background levels than the tritiated-thymidine method. But the staining method is useful for diverse cell types and the results attained through the staining technique were similar to those attained through the radiolabeling technique. The staining method produced reliable results in the screening of laminin and fibronectin peptides more quickly, less expensively, and less hazardously than the tritiated-thymidine labeling technique.
Sponsors
Degree Awarded
Bachelor's
Semester
Spring
Department
Life & Environmental Sciences