A Simple Staining Procedure for Quantifying Cell Adhesion in a Microtiter System

Abstract

The present study was designed to find a simple and effective staining technique to replace the tritiatedthymidine method in cell adhesion assays in 96-well microtiter plates. This method, a variation of previously used staining methods (Cardarelli and Pierschbacher, 1986; Barer, Lyon, and Draser, 1986), produced higher non-cellspecific background levels than the tritiated-thymidine method. But the staining method is useful for diverse cell types and the results attained through the staining technique were similar to those attained through the radiolabeling technique. The staining method produced reliable results in the screening of laminin and fibronectin peptides more quickly, less expensively, and less hazardously than the tritiated-thymidine labeling technique.