Molecular Cloning and Characterization of a 35 K Protein-Coding Gene that is Coregulated with the Gene(s) for the Outer Membrane Protein P.lla in Neisseria Gonorrhoeae

Abstract

A gene which coregulates the gene(s) that codes for the outer membrane protein P.ll in the bacterium Neisseria gonorrhoeae was cloned and characterized. As the cell expresses the P.ll protein, it no longer expresses the second protein; however, as the cell turns off the expression of P.ll, it then makes the second protein. A library of strain MS11 of the bacterium was constructed using the Lambda ZAP bacteriophage vector. This library was screened using a monospecific antiserum for the second protein. Molecular characterization of the positive clones followed.