Molecular Cloning and Characterization of a 35 K Protein-Coding Gene that is Coregulated with the Gene(s) for the Outer Membrane Protein P.lla in Neisseria Gonorrhoeae

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Authors
Wells, Kathryn
Advisor
John Christenson
Jean Smith
Tomas Graman
Editor
Date of Issue
1989-04-01
Subject Keywords
Publisher
Citation
Series/Report No.
item.page.identifier
Title
Molecular Cloning and Characterization of a 35 K Protein-Coding Gene that is Coregulated with the Gene(s) for the Outer Membrane Protein P.lla in Neisseria Gonorrhoeae
Other Titles
Type
thesis
Description
Abstract
A gene which coregulates the gene(s) that codes for the outer membrane protein P.ll in the bacterium Neisseria gonorrhoeae was cloned and characterized. As the cell expresses the P.ll protein, it no longer expresses the second protein; however, as the cell turns off the expression of P.ll, it then makes the second protein. A library of strain MS11 of the bacterium was constructed using the Lambda ZAP bacteriophage vector. This library was screened using a monospecific antiserum for the second protein. Molecular characterization of the positive clones followed.
Sponsors
Degree Awarded
Bachelor's
Semester
Spring
Department
Life & Environmental Sciences