The Effects of Tetrahydrocannabinol on Metabolism in Lithobates pipiens
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Authors
Magera, Heather
Mannalang, Carmen
Date of Issue
2025-04-25
Type
Presentation
Language
en_US
Subject Keywords
Other Titles
Abstract
11-Hydroxy-Tetrahydrocannabinol (11-OH-THC) is the primary psychoactive metabolite of Tetrahydrocannabinol (THC), a compound found in Cannabis. The effect of THC of mammalian vertebrates has been well studied, but the effect on amphibian physiology remains largely unexplored. The endocannabinoid system (ECS) has receptors that bind to THC, specifically CB1 and CB2 receptors, which play a critical role in modulating physiological processes, including metabolism, immune response, and cardiovascular function. The presence of CB1 receptors have been found in amphibians but the presence of CB2 is unknown. This study aims to investigate the impact of 11-OH-THC on standard metabolic rate at 20°C in leopard frogs (Lithobates pipiens). The experimental group will be dosed with 0.5 mg THC per kg frog and the control group will be ‘sham’ injected with saline. The exposure will be followed by metabolic rate measurements. Data will be statistically analyzed to determine if there are significant differences between groups. Findings from this research may contribute to a better understanding of cannabinoid receptor functions in non-mammalian vertebrates. Amphibians are ectothermic organisms and their metabolic rate is highly sensitive to environmental changes, making them an ideal model to study the role of THC on metabolic regulation. By exploring metabolic responses of amphibians to Cannabis, this research will provide valuable insights into the evolutionary conservation of ECS functions. Ultimately, this study aims to contribute to a more comprehensive understanding of cannabinoid expression and its impact on vertebrate physiology, specifically metabolic regulation, bridging gaps between cannabinoid research in mammalian and non-mammalian species.