Preliminary Characterization of Three Populations of the Freshwater Sponge, Clypeatula cooperensis, using RAPD-PCR

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Authors
Williams, Kathryn
Advisor
John Addis
Linda Beischel
Gerald Shields
Editor
Date of Issue
2000-04-01
Subject Keywords
freshwater sponge, Clypeatula cooperensis
Publisher
Citation
Series/Report No.
item.page.identifier
Title
Preliminary Characterization of Three Populations of the Freshwater Sponge, Clypeatula cooperensis, using RAPD-PCR
Other Titles
Type
thesis
Description
Abstract
In order to relate the three known populations of the freshwater sponge, Clypeatula cooperensis, to one another, sponge DNA was subjected to RAPDPCR. DNA was extracted using a Puregene kit from three specimens collected from each population. Following PCR optimization, DNA was amplified using random primers (OPA-10, 11, 12, 13, 18, and 20). Although reproducibility was a problem, preliminary results suggest that variability within populations is as high as variability among populations, raising the possibility that the three populations are genetically identical. This is an unexpected finding because one population is geographically isolated from the other two. If correct, one explanation is that the three populations have only recently become separated.
Sponsors
Degree Awarded
Bachelor's
Semester
Spring
Department
Life & Environmental Sciences