In Vitro Study Of The Effect Of Hypothermia On The Dissociation Curve Of Blood

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Munding, Matthew

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1992-04-01

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The aim of this study was to examine the change in the dissociation curve of oxygen from human hemoglobin at the temperatures of 37°, 25°, 18°, 12°, 7°, and 3° C. The partial pressures of oxygen and the calculated hemoglobin saturations were determined for samples at the various temperatures. From this data the partial pressures at 75% saturation were calculated, and the differences in the partial pressures at 75% saturation were compared. The dissociation curve was shown to shift to the left as the temperature was decreased down to 12°. Below 12°, the shift was not further observed. At the lower temperatures, the oxygen did not dissociate as well as it did at the higher temperatures.

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