The Detection Of Glycoproteins In The Plasma Membrane Of Dictyostelium discoideum

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Authors
Wood, Chris
Advisor
John Christenson
Rev. Jeremiah Lowney
Rev. Joseph Harrington
Editor
Date of Issue
1992-04-01
Subject Keywords
Dictyostelium discoideum
Publisher
Citation
Series/Report No.
item.page.identifier
Title
The Detection Of Glycoproteins In The Plasma Membrane Of Dictyostelium discoideum
Other Titles
Type
thesis
Description
Abstract
A clone of Dictyostelium discoideum strain AX-3 was cultured up to its amoeboid stage. The glycoproteins in the plasma membrane of strain AX-3 were then isolated and purified. The proteins were fractionated by SDS polyacrylamide gel electrophoresis and then transferred to a nitrocellulose sheet by western blotting. The resulting nitrocellulose blot was used to detect glycoproteins with biotinylated concanavalin A and avidin-alkaline phosphatase. The results suggest there are mannose containing glycoproteins in the plasma membrane of strain AX-3.
Sponsors
Degree Awarded
Bachelor's
Semester
Spring
Department
Life & Environmental Sciences