The Detection Of Glycoproteins In The Plasma Membrane Of Dictyostelium discoideum
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Authors
Wood, Chris
Date of Issue
1992-04-01
Type
thesis
Language
Subject Keywords
Dictyostelium discoideum
Other Titles
Abstract
A clone of Dictyostelium discoideum strain AX-3 was cultured up to its amoeboid stage. The glycoproteins in the plasma membrane of strain AX-3 were then isolated and purified. The proteins were fractionated by SDS polyacrylamide gel electrophoresis and then transferred to a nitrocellulose sheet by western blotting. The resulting nitrocellulose blot was used to detect glycoproteins with biotinylated concanavalin A and avidin-alkaline phosphatase. The results suggest there are mannose containing glycoproteins in the plasma membrane of strain AX-3.