Immunohistochemical Localization of Tubulin in the Somite of the Early Chick Embryo

carrollscholars.legacy.contextkey12080108
carrollscholars.legacy.itemurlhttps://scholars.carroll.edu/lifesci_theses/294
carrollscholars.object.degreeBachelor's
carrollscholars.object.departmentLife & Environmental Sciences
carrollscholars.object.disciplinesDevelopmental Biology
carrollscholars.object.seasonSpring
dc.contributor.advisorJohn Addis
dc.contributor.advisorJohn Christenson
dc.contributor.advisorJoan Stottlemyer
dc.contributor.authorScharfe, Stace
dc.date.accessioned2020-04-30T10:01:53Z
dc.date.available2020-04-30T10:01:53Z
dc.date.embargo12/31/1899 0:00
dc.date.issued1991-04-01
dc.description.abstractIn this experiment I attempted to devise a procedure for the localization of the cytoskeletal protein tubulin in the somite of the early chick embryo. I also checked the distribution of tubulin in the early chick somite for any possible relationship to the epithelial-mesenchymal transition that occurs in these somitic ceils. Newly fertilized chicken eggs were incubated for approximately 48 hr; then the embryos were removed from the egg, fixed, embedded, and frozen for cryostat sectioning. After sectioning the embryos, I attempted to localize tubulin by attaching a primary antibody to the tubulin molecule followed by a secondary antibody and an enzyme (peroxidase). A peroxidase substrate was then introduced to the tissue, and the enzyme reacted with it to produce a colored reaction product which served as the indicator for the presence and distribution of the tubulin.
dc.identifier.urihttps://scholars.carroll.edu/handle/20.500.12647/3036
dc.titleImmunohistochemical Localization of Tubulin in the Somite of the Early Chick Embryo
dc.typethesis
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