Studies of Mosaicism for Chromosomal Aneuploidy in Fetal Loss Using Fluorescence In Situ Hybridization on Several Tissue Types

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Authors
Knox, Aaron
Advisor
Marilyn Schendel
Mary Haag
John Addis
Editor
Date of Issue
1999-04-01
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Citation
Series/Report No.
item.page.identifier
Title
Studies of Mosaicism for Chromosomal Aneuploidy in Fetal Loss Using Fluorescence In Situ Hybridization on Several Tissue Types
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Type
thesis
Description
Abstract
The main objective ofthis study was to determine the presence and level of mosaicism for chromosomal aneuploidy in several cases of fetal demise that had previous indications of chromosome aberrations. The presence of aneuploidy was determined using fluorescence in situ hybridization (FISH) probes. Techniques that were utilized in attempts to prepare several tissue types for FISH are described. The analyses made with FISH provided useful data on the chromosomal makeup oftwo of the cases studied. In one case FISH results showed that the product of conception (POC) did not contain the trisomy 18 that is present at low levels in the mother’s blood cells. In the other case the miscarried fetus was previously identified as being mosaic for trisomy X. In this research project the presence ofvarying levels of trisomy X in both embryonic and placental tissue was confirmed
Sponsors
Degree Awarded
Bachelor's
Semester
Spring
Department
Life & Environmental Sciences