Preliminary Characterization Of The P3 Human Epithelial Cell Line For Radiobiological Reseach

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Authors
Holland, John
Advisor
Rev. Joseph Harrington
James Manion
Rev. Robert Butko
Editor
Date of Issue
1984-04-01
Subject Keywords
Publisher
Citation
Series/Report No.
item.page.identifier
Title
Preliminary Characterization Of The P3 Human Epithelial Cell Line For Radiobiological Reseach
Other Titles
Type
thesis
Description
Abstract
Preliminary experimentation was performed to characterize the P3 human epithelial cell line for radiobiological research. Specifically, work was done to determine if the cell line should be used to evaluate mechanisms of radiation damage and repair as revealed by survival response experimentation and via the 6-thioguanine mutation assay. Growth analysis was performed as well as a 6-thioguanine toxicity study and a 6-thioguanine mutation expression time curve. Preliminary results were obtained from survival experiments following P3 cell irradiation with 50 KvP X-rays, 6^co gamma rays, and Janus Reactor fission spectrum neutrons with a mean energy of 0.86 MeV. These and preliminary results of 6-thioguanine resistance mutation frequency experiments utilizing Janus Reactor fission spectrum neutrons will be discussed.
Sponsors
Degree Awarded
Bachelor's
Semester
Spring
Department
Life & Environmental Sciences