Studies On The Effect OF Azaserine On DNA

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Authors
Zucker, Charles
Advisor
John Christenson
Guido Bugni
Rev. Joseph Harrington
Editor
Date of Issue
1981-04-01
Subject Keywords
Publisher
Citation
Series/Report No.
item.page.identifier
Title
Studies On The Effect OF Azaserine On DNA
Other Titles
Type
thesis
Description
Abstract
Azaserine (O-diazoacetyl-L-serine) is known to be a non-competitive inhibitor of two enzymes necessary for de novo purine biosynthesis. Work done in this laboratory and others, however, indicates that much of azaserine’s effect in cytotoxicity, mutagenesis and carcinogenesis can be attributed to its action on DNA. DNA repair-deficient strains of Escherichia coli are found to be more sensitive to azaserine cytotoxicity and, in cases, to azaserine mutagenesis than the wild type E. coli. Attempts at showing an in vitro effect of azaserine on DNA have not been conclusive as of yet. An interesting aspect of azaserine cytotoxicity is that it can be partially reversed by growth in low levels of the alkylating agent N-methyl-N'-nitro-N-nitroso-guanidine. This adaptive response suggests that part of the in vivo action of azaserine involves an alkylation of the DNA.
Sponsors
Degree Awarded
Bachelor's
Semester
Spring
Department
Life & Environmental Sciences