The Effect of PKC 5 Inhibitors on PMA-induced Apoptosis in Xenopus laevis Splenocytes
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Authors
Crater, Annie
Advisor
Gerald Shields
Grant Hokit
Ron Wilde
Grant Hokit
Ron Wilde
Editor
Date of Issue
2003-04-01
Subject Keywords
Publisher
Citation
Series/Report No.
item.page.identifier
Title
The Effect of PKC 5 Inhibitors on PMA-induced Apoptosis in Xenopus laevis Splenocytes
Other Titles
Type
thesis
Description
Abstract
Protein kinase C (PKC) controls apoptosis in amphibian systems such as that of the South African clawed frog, Xenopus laevis. The effects of cancer inducing agents like phorbol 12-myristate 13-acetate (PMA) when combined with Ca2+ independent PKC inhibitors on programmed cell death were studied in A laevis splenocytes. Regulation of PMA-activated cells was observed via Annexin V-FITC and propidium iodide assays, flow cytometry, and Western blot analysis. Results indicate the PKC inhibitors reduced both apoptosis and cell growth in PMA-stimulated X. laevis splenocytes compared to PMA alone. These results are valuable to future mammalian cancer research. By identifying the various apoptotic mechanisms utilized by amphibian cells, researchers may become more informed regarding amphibians’ increased resistance to cancer and apply such knowledge to mammalian systems.
Sponsors
Degree Awarded
Bachelor's
Semester
Spring
Department
Life & Environmental Sciences