The Effects of Intermittent Fasting Diet at Different Stages of Circadian Rhythm on Mus musculus on Metabolic Rate, Glucose and Ketone Levels, and Overall Body Fat Loss
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Authors
Lohr, Kelsyn
Date of Issue
2023-04-28
Type
Presentation
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Abstract
Intermittent fasting (IF) diets are popular weight loss regimens that are thought to promote heart health, fight inflammation, and reduce risk of cancer. For humans, fasting periods typically take place during the night with feeding periods during the day. However, it is unknown if this is the best fasting method in relation to a mammal’s circadian rhythm. Using laboratory mice (Mus musculus), we will study the efficacy of the IF diets at different points within a daily circadian rhythm. Mice will be placed in three groups: mice feeding ad libitum, mice fasting overnight, and mice fasting during the day. Metabolic rate, respiratory quotient, urine glucose and ketone levels, and overall weight change of the three groups will be quantified over a seven week period. Glucose and ketone levels will be tested using 96 well colorimetric assay kits. A flow-through respirometer will be used to test metabolic rate and respiratory quotient. If the time of day at which fasting occurs results in changes in lipid metabolism then we should observe significant differences in metabolic rate, respiratory quotient, urine glucose and ketone levels, and body weight between the daytime and nighttime fasted mice.