Metal Ion And Penicillin Resistance In Staphylococcus Aureus

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Authors
Forbes, Andrew
Marosok, Randall
Advisor
James Manion
Arthur Westwell
Alan Barbour
Editor
Date of Issue
1981-04-01
Subject Keywords
Publisher
Citation
Series/Report No.
item.page.identifier
Title
Metal Ion And Penicillin Resistance In Staphylococcus Aureus
Other Titles
Type
thesis
Description
Abstract
An agar dilution test and a disc diffusion test were developed for determining lead susceptibility of Staphylococcus aureus. An agar medium with 0.008 molar concentrations each of lead acetate and EDTA was found to be reliable for screening lead ion resistant strains of S. aureus. A test using six mm discs of Whatman #3 filter paper impregnated with 20 ul of 0.2 M lead acetate was also found to be adequate for determining lead resistance of S. aureus. We found that in this test the acetate does not precipitate into the medium, yet it gives a definitive test for lead ion resistance. A total of 113 strains of S. aureus were screened for resistance to cadmium, arsenate, arsenite, lead, bismuth, and inorganic mercury ions as well as to penicillin (10 units) by the disc diffusion method. We found that many of these resistances were linked because they frequently appeared together among all the strains of S. aureus. On the other hand, there was significant variability among all the strains. Finally, our data support the existing theories of staphylococcal plasmid evolution.
Sponsors
Degree Awarded
Bachelor's
Semester
Spring
Department
Life & Environmental Sciences