The Isolation And Quantification Of Noradrenalin And The Effects of 6-Hydroxydopamine On The Noradrenalin Levels Of The Cerebral Cortex Of Mice

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Authors
Roth, Bryan
Advisor
John Christenson
Guido Bugni
James Manion
Editor
Date of Issue
1977-04-01
Subject Keywords
Publisher
Citation
Series/Report No.
item.page.identifier
Title
The Isolation And Quantification Of Noradrenalin And The Effects of 6-Hydroxydopamine On The Noradrenalin Levels Of The Cerebral Cortex Of Mice
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Type
thesis
Description
Abstract
Noradrenalin was isolated on a Dowex-50X8 cation-exchange resin and quantified by the trihydroxyindole technique of fluorometry. A comparison of column dimensions showed a longer, thinner column was significantly more efficient in isolating noradrenalin. Subcutaneous injections of 6-OHDA were shown to significantly decrease the noradrenalin levels of the cerebral cortex of neonatal and adult mice. However, no effect was noted on mice injected as infants and allowed to mature. This suggests that the effects of 6-OHDA are transient at best, and that the blood-brain barrier to catecholamines is not as effective in mice as rats. Discussion of the limitations of the procedure is included.
Sponsors
Degree Awarded
Bachelor's
Semester
Spring
Department
Life & Environmental Sciences